[2020] Backbone NMR assignments of the anti-CRISPR AcrIIA5 from phages infecting Streptococcus thermophiles
Journal
J Korean Mag Res Soc 24(3): 70-76
Authors
An, S.Y., Kim, E.H., Bae, E. and Suh, J.Y.*
*Denotes Corresponding Author
Abstract
Journal
J Korean Mag Res Soc 24(3): 70-76
Authors
An, S.Y., Kim, E.H., Bae, E. and Suh, J.Y.*
*Denotes Corresponding Author
Abstract
The CRISPR-Cas system provides an adaptive immunity for bacteria and archaea against invading phages or foreign plasmids. In the type II CRISPR-Cas system, a single effector protein Cas9 and a guide RNA form an RNA-guided endonuclease complex that can degrade DNA targets of foreign origin. To avoid the Cas9-mediated destruction, phages evolved anti-CRISPR (Acr) proteins that neutralize the host bacterial immunity by inactivating the CRISPR-Cas system. Here we report the backbone 1H, 15N, and 13C resonance assignments of AcrIIA5 that inhibits the endonuclease activity of type II-A Streptococcus thermophilus Cas9 and also Streptococcus pyogenesis Cas9 using triple resonance nuclear magnetic resonance spectroscopy. The backbone chemical shifts of AcrIIA5 predict a disordered region at the N-terminus, followed by an alpha-beta-beta-beta-beta-alpha-beta-beta-beta fold.